Linking Ubiquitin Research to Drug Discovery

Is the abundancy of my protein of interest regulated by the ubiquitin system? What happens to the abundancy of my protein of interest if a component of the ubiquitin system is knocked out or mutated in a site directed manner such as a; 1) DUB catalytic site mutation, 2) full DUB KO, 3) KO of an E3 ligase or a sub-component of an E3 ligase such as a substrate binding adaptor, 4) KO of an E2 conjugating enzyme?

Monitor up or down regulation of your protein of interest in the ubiquitin system modified relative to the control WT HAP1 cell line.

Readout methods may include for example; 1) Western blotting if an antibody is available, 2) tagging your protein of interest with a fluorescent protein and monitoring changes in fluorescence.

Is the localisation of my protein of interest regulated by the ubiquitin system? What happens to the localisation of my protein of interest if a component of the ubiquitin system is knocked out or mutated in a site directed manner as above?

Monitor the sub-cellular localisation and/or protein/protein interactions of your protein of interest tagged with a fluorescent protein.

Is my cell phenotype of interest regulated by the ubiquitin system? What happens to a phenotype of the HAP1 cell line (in the presence and/or absence of other ligands such as growth factors) if a component of the ubiquitin system is knocked out or mutated in a site directed manner as above?

We can transfer your phenotypic assay of choice to Ubiquigent and monitor the outcome of addressing this question using modified and control   HAP1 cell lines.