Linking Ubiquitin Research to Drug Discovery

Validation and Experimental Utility of the E2scan™ Kit v2

Posted in Resource categoriesApplication Notes for Kits and Services.
Attachment: 1 Publication

The E2scan Kit version 2 is designed to facilitate the identification of E2 conjugating enzymes which support the ubiquitylation of substrate proteins catalysed by an E3 ligase and/or the auto-ubiquitylation of the E3 ligase itself.

The kit contains an E2scan plate (a panel of 34 E2 conjugating enzymes arrayed in duplicate across a 96 well plate) plus all the components (E1 enzyme, control E3 ligase, ubiquitin, ATP and assay buffer) required to perform a complete substrate ubiquitylation or auto-ubiquitylation assay using your E3 ligase and/or E3 ligase/substrate combination of choice.

Publication:
  • Validation and Experimental Utility of the E2scan™ Kit version 2
    Validation and Experimental Utility of the E2scan™ Kit version 2