The anti-polyubiquitylated conjugates mAb (FK2) demonstrate specific recognition of polyubiquitylated and monoubiquitylated proteins but shows no reactivity with free ubiquitin (Fujimoro et al. 1994). The anti-polyubiquitylated conjugates mAb (FK2) has been extensively characterised and used not only to investigate ubiquitin chain formation on poly and mono ubiquitylated proteins by Western blotting but also in the detection of intracellular polyubiquitin chains in immunoassays (Takada et al. 1995; Fujimoro et al. 2005).
Fujimuro M, Sawada H, Yokosawa H (1994) Production and characterization of monoclonal antibodies specific to multiubiquitin chains of polyubiquitinated proteins. FEBS Lett 349173-180.
Takada K, Nasu H, Hibi N, Tsukada Y, Ohkawa K, Fujimuro M, Sawada H, Yokosawa H (1995) Immunoassay for the quantification of intracellular multi-ubiquitin chains. Eur J Biochem 233 42-47.
Fujimuro M, Yokosawa H (2005) Production of antipolyubiquitin monoclonal antibodies and their use for characterization and isolation of polyubiquitinated proteins. Methods Enzymol 39975-86.