Linking Ubiquitin Research to Drug Discovery

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  • Name
    Catalogue Number
    Size
    Price
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  • Name:
    USP25 [GST-tagged]
    Catalogue Number:
    64-0023-050
    Size:
    50 µg
    Price:
    £195
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  • Species
    human
  • Source
    E.coli
  • Quantity
    50 µg
  • Storage
    -70°C
  • Concentration
    0.5 mg/ml
  • Formulation
    50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
  • Molecular Weight
    ~149 kDa
  • Stability
    12 months at -70°C; aliquot as required
  • Protein Sequence
    Accession number: NP_037528. For full protein sequence information download the Certificate of Analysis pdf.
  • QA; Protein Identification
    Confirmed by mass spectrometry.
  • QA; Activity
    Deubiquitylase Enzyme Assay: The activity of GST-USP25 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of GST-USP25 was compared confirming the deubiquitylating activity of GST-USP25.

Deconjugating enzymes (DCEs) are proteases that process ubiquitin or ubiquitin-like gene products, reverse the modification of proteins by a single ubiquitin or ubiquitin-like protein (UBL) and remodel polyubiquitin (or poly-UBL) chains on target proteins (Reyes-Turcu et al., 2009). The deubiquitylating – or deubiquitinating – enzymes (DUBs) represent the largest family of DCEs and regulate ubiquitin dependent signaling pathways. The activities of the DUBs include the generation of free ubiquitin from precursor molecules, the recycling of ubiquitin following substrate degradation to maintain cellular ubiquitin homeostasis and the removal of ubiquitin or ubiquitin-like proteins (UBL) modifications through chain editing to rescue proteins from proteasomal degradation or to influence cell signalling events (Komander et al., 2009). There are two main classes of DUB, cysteine proteases and metalloproteases. Ubiquitin carboxyl-terminal hydrolase 25 (Ubiquitin Specific Protease 25; USP25) is a member of the cysteine protease enzyme family and cloning of the human gene was first described by Valero et al. (1999).  USP25 was originally called USP21 as it was found on chromosome 21, region 21q11-q21. The chromosome 21 trisomy (Down syndrome) is the most frequent human birth defect, and an increase in USP25 gene dosage in Down syndrome patients could seriously disturb the balance between ubiquitylated and deubiquitylated substrates (Valero et al., 1999). Instability and allelic deletions of the 21q11-q21 region of the chromosome have been found in 13 of 34 squamous non-small cell lung carcinomas (Groet et al., 2000).  USP25 is a target for SUMOylation, being more efficient with SUMO2/3. USP25 SUMOylation impairs binding to and hydrolysis of ubiquitin chains (Meulmeester et al., 2008). USP25 is deSUMOylated by SENP1 (Mohideen and Lima, 2008).
References:

Groet J, Ives JH, Jones TA, Danton M, Flomen RH, Sheer D, Hrascan R, Pavelic K, Nizetic D (2000) Narrowing of the region of allelic loss in 21q11-21 in squamous non-small cell lung carcinoma and cloning of a novel ubiquitin-specific protease gene from the deleted segment. Genes Chromosomes Cancer 27, 153-161.

Komander D, Clague MJ, Urbe S (2009) Breaking the chains: structure and function of the deubiquitinases. Nat Rev Mol Cell Biol 10, 550-563.

Meulmeester E, Kunze M, Hsiao HH, Urlaub H, Melchior F (2008) Mechanism and consequences for paralog-specific sumoylation of ubiquitin-specific protease 25. Mol Cell 30, 610-619.

Mohideen F, Lima CD (2008) SUMO takes control of a ubiquitin-specific protease. Mol Cell 30, 539-540.

Reyes-Turcu FE, Ventii KH, Wilkinson KD (2009) Regulation and cellular roles of ubiquitin-specific deubiquitinating enzymes. Ann Rev Biochem 78, 363-397.

Valero R, Marfany G, Gonzalez-Angulo O, Gonzalez-Gonzalez G, Puelles L, Gonzalez-Duarte R (1999) USP25, a novel gene encoding a deubiquitinating enzyme, is located in the gene-poor region 21q11.2. Genomics 62, 395-405.