*Purity of all products >95% unless otherwise stated
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NameCatalogue NumberSizePriceAdd to Basket
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Catalogue Number:66-3002-010Size:10 mgPrice:£250Add To Basket
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SpeciesHuman
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SourceHuman Embryonic kidney
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Quantity10 mg
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Storage-70°C
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Concentration20mg/ml
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Formulation50mM Tris/HCl pH7.5, 1mM EGTA, 1mM EDTA, 1% Triton X-100, 1mM sodium orthovanadate, 50mM sodium fluoride, 5mM sodium pyrophosphate, 10mM sodium β-glycerophosphate, 270mM sucrose, 1mM PMSF, 1mM benzamidine, 50mM iodoacetamide.
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Molecular Weightn/a
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Stability12 months at -70°C; aliquot as required
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Protein Sequencen/a
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QA; Protein Identificationn/a
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QA; ActivityIdentification of Polyubiquitin Chains in Lysate: A Western blot of IL-1 stimulated HEK293 cell lysate (lane 2) and non-stimulated HEK293 cell lysate (lane 3) probed with an anti-FK2 antibody indicates that both of the lysates are abundant with ubiquitylated proteins and possibly free ubiquitin chains. Polyubiquitin (Ub2-7) (K63-linked) was included as a positive control in this experiment (lane 1).
HEK293 cell lysate may be used to demonstrate the ability of ubiquitin binding domain proteins (Op tineurin and NEMO) to capture polyubiq uitylated proteins including IL-1 receptor-associated kinase 1 (IRAK1) (Windheim et al.2008).
Reference:
Windheim M, Stafford M, Peggie M, Cohen P (2008) Interleukin-1 (IL-1) induces the Lys63-linked polyubiquitination of IL-1 receptor-associated kinase 1 to facilitate NEMO binding and the activation of IkappaBalpha kinase. Mol Cell Biol 28, 1783-91.