Background
HEK293 Cell Lysate (IL-1 stimulated) may be used to demonstrate the ability of ubiquitin binding domain proteins (Optineurin and NEMO) to capture polyubiquitylated proteins including IL-1 receptor-associated kinase 1 (IRAK1) (Windheim et al. 2008).
References
Windheim M, Stafford M, Peggie M, Cohen P (2008) Interleukin-1 (IL-1) induces the Lys63-linked polyubiquitination of IL-1 receptor-associated kinase 1 to facilitate NEMO binding and the activation of IkappaBalpha kinase. Mol Cell Biol 28, 1783-91.