*Purity of all products >95% unless otherwise stated
-
NameCatalogue NumberSizePriceAdd to Basket
-
Catalogue Number:62-0017-100Size:100 µgPrice:£325Add To Basket
-
SpeciesHuman
-
SourceE. coli expression
-
Quantity100 μg
-
Storage-70°C
-
Concentration1 mg/ml
-
Formulation50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
-
Molecular Weight~20 kDa
-
Stability12 months at -70°C; aliquot as required
-
Protein SequenceAccession number: NP_057067. For full protein sequence information download the Certificate of Analysis pdf.
-
QA; Protein IdentificationConfirmed by mass spectrometry.
-
QA; ActivityE2-Ubiquitin Thioester Loading Assay: The activity of UBE2D4 was validated by loading E1 UBE1 activated ubiquitin onto the active cysteine of the UBE2D4 E2 enzyme via a transthiolation reaction. Incubation of the UBE1 and UBE2D4 enzymes in the presence of ubiquitin and ATP at 30°C was compared at two time points, T0 and T10 minutes. Sensitivity of the ubiquitin/UBE2D4 thioester bond to the reducing agent DTT was confirmed.
The enzymes of the ubiquitylation pathway play a pivotal role in a number of cellular processes including regulated and targeted proteasomal degradation of substrate proteins. Three classes of enzymes are involved in the process of ubiquitylation; activating enzymes (E1s), conjugating enzymes (E2s) and protein ligases (E3s). UBE2D4 is a member of the E2 ubiquitin-conjugating enzyme family and the human gene was first described by Colland et al. (2004).
References:
Colland F, Jacq X, Trouplin V, Mougin C, Groizeleau C, Hamburger A, Meil A, Wojcik A, Legrain P, Gauthier J (2004) Functional proteomics mapping of a human signaling pathway. Genome Res 14, 1324-32.