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NameCatalogue NumberSizePriceAdd to Basket
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Catalogue Number:62-0028-020Size:20 µgPrice:£100Add To Basket
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SpeciesHuman
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SourceE. coli expression
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Quantity20 μg
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Storage-70°C
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Concentration1 mg/ml
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Formulation50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
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Molecular Weight~46 kDa
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Stability12 months at -70°C; aliquot as required
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Protein SequenceAccession number: NP_003333. For full protein sequence information download the Certificate of Analysis pdf.
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QA; Protein IdentificationConfirmed by mass spectrometry.
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QA; ActivityE2-Ubiquitin Thioester Loading Assay: The activity of GST-UBE2G1 was validated by loading E1 UBE1 activated ubiquitin onto the active cysteine of the GST-UBE2G1 E2 enzyme via a transthiolation reaction. Incubation of the UBE1 and GST-UBE2G1 enzymes in the presence of ubiquitin and ATP at 30oC was compared at two time points, T0 and T10 minutes. The sensitivity of this ubiquitin/GST-UBE2G1 thioester bond to the reducing agent DTT was confirmed.
The enzymes of the ubiquitylation pathway play a pivotal role in a number of cellular processes including regulated and targeted proteasomal degradation of substrate proteins. Three classes of enzymes are involved in the process of ubiquitylation; activating enzymes (E1s), conjugating enzymes (E2s) and protein ligases (E3s). UBE2G1 is a member of the E2 conjugating enzyme family and cloning of the human gene was first described by Watanabe et al. (1996). UBE2G1 shares 74% sequence identity with UBC7 from C. elegans and a high degree of homology with UBC7 from other species. Expression of UBE2G1 and a helix-loop-helix transcription factor and member of the MYC/MAX superfamily (ROX/MNT) is decreased in medullablastoma tumours. Haploinsufficiency of the human 17p13.3 region is associated with 35% to 50% of medullablastomas, indicating the presence of one or more tumour suppressor genes which have not yet been identified (Cvekl et al., 2004).
References:
Cvekl A, Jr., Zavadil J, Birshtein BK, Grotzer MA, Cvekl A (2004) Analysis of transcripts from 17p13.3 in medulloblastoma suggests ROX/MNT as a potential tumour suppressor gene. Eur J Cancer 40, 2525-32.
Watanabe TK, Kawai A, Fujiwara T, Maekawa H, Hirai Y, Nakamura Y, Takahashi E (1996) Molecular cloning of UBE2G, encoding a human skeletal muscle-specific ubiquitin-conjugating enzyme homologous to UBC7 of C. elegans. Cytogenet Cell Genet 74, 146-8.