Enabling Protein Degradation Drug Discovery

human

E.coli

50 µg

-70°C

0.5 mg/ml

50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol

~128kDa

12 months at -70°C; aliquot as required

Accession number: NP_005145. For full protein sequence information download the Certificate of Analysis pdf.

Confirmed by mass spectrometry.

Deubiquitylase Enzyme Assay: The activity of USP8 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of USP8 was compared confirming the deubiquitylating activity of USP8.