4x Ubiquitin-Rhodamine 110


Catalogue Number
60-0122-500
Product Size
500 µl
Price £
£200
Certificate of Analysis Size
500 µl
Species
human
Source
synthetic
Quantity
500 µl
Storage
-70°C
Concentration
0.4µM
Formulation
DTT containing buffer
Molecular Weight
~8.93kDa
Stability
12 months at -70°C. Avoid multiple freeze/thaw cycles.
Protein Sequence
Accession number: P62987. C-terminally tagged with Rhodamine 110
QA; Protein Identification
Confirmed by mass spectrometry.
QA Activity

Activity Assay: The activity of Ubiquitin-Rhodamine 110 was validated by determining the increase in fluorescence at 535nm (Excitation 485nm) measured as a result of the enzyme catalysed cleavage at the amide bond between the C-terminal Glycine and Rhodamine, generating Ubiquitin and de-quenched Rhodamine 110-Glycine. UCHL3 (deubiquitylase) was incubated with Ubiquitin-Rhodamine 110 and the fluorescence was measured at four time points (0min, 30min, 60min and 90min).


Background

see Certificate of Analysis