USP1 [6His-tagged]/UAF1 [untagged]


Catalogue Number
64-0040-050
Product Size
50 µg
Price £
£325
Accession Number
NP_003359/NP_065890
Residues Expressed
1-785/1-677
Certificate of Analysis Size
50 µg
Species
human
Source
Insect sf21
Quantity
50 µg
Storage
-70°C
Concentration
0.5 mg/ml
Formulation
50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Molecular Weight
USP1 = 90.5 kDa/UAF1 = 76.2 kDa
Stability
12 months at -70°C; Ubiquigent strongly recommends aliquoting for single use as required
Protein Sequence
Accession number: USP1 = NP_003359 and UAF1 = NP_065890. For full protein sequence information download the Certificate of Analysis pdf.
QA; Protein Identification
Confirmed by mass spectrometry.
QA Activity

Deubiquitylase Enzyme Assay: The activity of 6His-USP1/UAF1 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of 6His-USP1/UAF1 was compared confirming the deubiquitylating activity of 6His-USP1/UAF1.


Background

Deconjugating enzymes (DCEs) are proteases that process ubiquitin or ubiquitin-like gene products, reverse the modification of proteins by a single ubiquitin or ubiquitin-like protein (UBL) and remodel polyubiquitin (or poly-UBL) chains on target proteins (Reyes-Turcu et al., 2009). The deubiquitylating - or deubiquitinating - enzymes (DUBs) represent the largest family of DCEs and regulate ubiquitin-dependent signalling pathways. The activities of the DUBs include the generation of free ubiquitin from precursor molecules, the recycling of ubiquitin following substrate degradation to maintain cellular ubiquitin homeostasis and the removal of ubiquitin or ubiquitin-like proteins (UBL) modifications through chain editing to rescue proteins from proteasomal degradation or to influence cell signalling events (Komander et al., 2009). There are two main classes of DUB, cysteine proteases and metalloproteases. Ubiquitin specific protease 1 (USP1) is a member of the cysteine protease enzyme family and cloning of the human gene was first described by Fujiwara et al. (1998). USP1 has been shown to act as a negative regulator of the Fanconi anaemia pathway. Inhibition of USP1 by siRNA knockdown increased the monoubiquitylation of the Fanconi anaemia effector protein, FANCD2, and increased cellular resistance to DNA cross-linking agents, suggesting that USP1 deubiquitylates FANCD2 (Huang et al., 2006). USP1 associated factor 1 (UAF1) not only interacts with and stabilizes USP1 but also stimulates its enzymatic activity. UAF1 binding increases the catalytic turnover (Kcat) but does not increase the affinity of the USP1 enzyme for the substrate (KM) (Cohn et al., 2007). To explore USP1 autocleavage, a USP1 variant was expressed where the amino acids on positions 670 and 671 were changed from Gly-Gly to Ala-Ala. This mutation inhibited both the cleavage and degradation of USP1 (Huang et al., 2006). USP1 plays important roles in cancer-related processes, such as the DNA damage response, and the maintenance of the undifferentiated state of osteosarcoma cells. Inhibiting the function of the USP1/UAF1 complex sensitizes cancer cells to chemotherapy, suggesting that this complex is a relevant anticancer target (Garcia-Santisteban et al., 2012).


References

Fujiwara T, Saito A, Suzuki M, Shinomiya H, Suzuki T, Takahashi E, et al. (1998) Identification and chromosomal assignment of USP1, a novel gene encoding a human ubiquitin-specific protease. Genomics, 54, 155-158.

Garcia-Santisteban I, Zorroza K and Rodriguez JA (2012) Two nuclear localization signals in USP1 mediate nuclear import of the USP1/UAF1 complex. PloS one, 7, e38570.

Huang TT, Nijman SM, Mirchandani KD, Galardy PJ, Cohn MA, Haas W, et al. (2006) Regulation of monoubiquitinated PCNA by DUB autocleavage. Nature Cell Biology, 8, 339-347.

Komander D, Clague MJ and Urbe S (2009) Breaking the chains: structure and function of the deubiquitinases. Nat Rev Mol Cell Biol, 10, 550-563.

Reyes-Turcu FE, Ventii KH and Wilkinson KD (2009) Regulation and cellular roles of ubiquitin-specific deubiquitinating enzymes. Annual Review of biochemistry, 78, 363-397.