ITCH [GST-tagged]

Catalogue Number
Product Size
25 µg
Price £
Accession Number
Residues Expressed
Certificate of Analysis Size
25 µg
E. coli expression
25 μg
0.5 mg/ml
50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Molecular Weight
~125.5 kDa
12 months at -70°C; aliquot as required
Protein Sequence
Accession number: NP_1136713. For full protein sequence information download the Certificate of Analysis pdf.
QA; Protein Identification
Confirmed by mass spectrometry.
QA Activity

E3 ligase assay: The ubiquitin conjugating activity of GST-ITCH was validated through its ability to catalyse the generation of polyubiquitin chains in the presence of the E1 activating enzyme His-UBE1, the E2 conjugating enzyme His-UBE2D3 (UbcH5c) (several E2s were tested, data generated with this E2 is provided by way of example) and ubiquitin. Incubation of GST-ITCH for 60 minutes at 37°C in the presence of ubiquitin, His-UBE1, His-UBE2D3 and ATP (Lane 1) was compared alongside two control reactions with either ATP (Lane 2) or GST-ITCH (Lane 3) excluded from the reaction. Ubiquitin conjugates were identified by Western blotting using an anti-ubiquitin conjugate antibody and these were observed only in the presence of both ATP and GST-ITCH (with the exception of one species of approximately 140 MW observable in lane 3).


The enzymes of the ubiquitylation pathway play a pivotal role in a number of cellular processes including the regulated and targeted proteasome-dependent degradation of substrate proteins. Three classes of enzymes are involved in the process of ubiquitylation; activating enzymes (E1s), conjugating enzymes (E2s) and protein ligases (E3s). Itchy E3 ubiquitin protein ligase (ITCH) is a member of the E3 protein ligase family and cloning of the human gene was first described by Wood et al. (1998). ITCH belongs to the HECT family of E3 ubiquitin-ligases that regulate key trafficking decisions, including targeting of proteins to the proteasome or lysosomes. ITCH protein contains an N-terminal C2 domain, four tandem WW domains and a HECT (homologous to the E6 associated protein carboxyl terminus) domain. ITCH has been shown to mediate the ubiquitylation of EGF and CXCR4 receptors, targeting them for degradation (Azakir and Angers 2009; Marchese et al., 2003). JNK phosphorylates ITCH activating its E3 ligase activity, which in turn controls the turnover of Jun proteins and T cell differentiation. The activity of ITCH ubiquitin ligase is negatively regulated by Fyn catalysed tyrosine phosphorylation of ITCH Tyr371 (Yang et al. 2006).


Azakir BA, Angers A (2009) Reciprocal regulation of the ubiquitin ligase Itch and the epidermal growth factor receptor signaling. Cell Signal 21, 1326-36.

Marchese A, Raiborg C, Santini F, Keen JH, Stenmark H, Benovic JL (2003) The E3 ubiquitin ligase AIP4 mediates ubiquitination and sorting of the G protein-coupled receptor CXCR4. Dev Cell 5, 709-22.

Wood JD, Yuan J, et al. (1998) Atrophin-1, the DRPLA gene product, interacts with two families of WW domain-containing proteins. Mol Cell Neurosci 11, 149-60.

Yang C, Zhou W, Jeon MS, Demydenko D, Harada Y, Zhou H, Liu YC (2006) Negative regulation of the E3 ubiquitin ligase itch via Fyn-mediated tyrosine phosphorylation. Mol Cell 21, 135-41.