HEK293 cell lysate (IL-1 stimulated)


Catalogue Number
66-3001-010
Product Size
10 mg
Price £
£325
Accession Number
n/a
Residues Expressed
n/a
Certificate of Analysis Size
10 mg
Species
Human
Source
IL-1-stimulated embryonic kidney (HEK293) cells
Quantity
10 mg
Storage
-70°C
Concentration
20mg/ml
Formulation
50mM Tris/HCl pH7.5, 1mM EGTA, 1mM EDTA, 1% Triton X-100, 1mM sodium orthovanadate, 50mM sodium fluoride, 5mM sodium pyrophosphate, 10mM sodium β-glycerophosphate, 270mM sucrose, 1mM PMSF, 1mM benzamidine, 50mM iodoacetamide.
Molecular Weight
n/a
Stability
12 months at -70°C; aliquot as required
Protein Sequence
n/a
QA; Protein Identification
n/a
QA Activity

Identification of Polyubiquitin Chains in Lysate: A Western blot of IL-1 stimulated HEK293 cell lysate (lane 2) and non-stimulated HEK293 cell lysate (lane 3) probed with an anti-FK2 antibody indicates that both of the lysates are abundant with ubiquitylated proteins and possibly free ubiquitin chains. Polyubiquitin (Ub2-7) (K63-linked) was included as a positive control in this experiment (lane 1).


Background

HEK293 Cell Lysate (IL-1 stimulated) may be used to demonstrate the ability of ubiquitin binding domain proteins (Optineurin and NEMO) to capture polyubiquitylated proteins including IL-1 receptor-associated kinase 1 (IRAK1) (Windheim et al. 2008).


References

Windheim M, Stafford M, Peggie M, Cohen P (2008) Interleukin-1 (IL-1) induces the Lys63-linked polyubiquitination of IL-1 receptor-associated kinase 1 to facilitate NEMO binding and the activation of IkappaBalpha kinase. Mol Cell Biol 28, 1783-91.