The anti-mono and polyubiquitylated conjugates mAb (FK2) demonstrates specific recognition for monoubiquitylated and polyubiquitylated proteins but shows no reactivity with free ubiquitin (Fujimoro et al. 1994). The anti-mono and polyubiquitylated conjugates mAb (FK2) has been extensively characterised and used not only to investigate ubiquitin chain formation on mono and polyubiquitylated proteins by Western blotting but also in the detection of intracellular polyubiquitin chains in immunoassays (Takada et al. 1995; Fujimoro et al. 2005). The biotin linked anti-mono and polyubiquitylated conjugates mAb (FK2) facilitates the detection of mono and polyubiquitylated conjugates with the use of a streptavidin HRP conjugate.
Fujimuro M, Sawada H, Yokosawa H (1994) Production and characterization of monoclonal antibodies specific to multiubiquitin chains of polyubiquitinated proteins. FEBS Lett 349 173-180.
Takada K, Nasu H, Hibi N, Tsukada Y, Ohkawa K, Fujimuro M, Sawada H, Yokosawa H (1995) Immunoassay for the quantification of intracellular multi-ubiquitin chains. Eur J Biochem 233 42-47.
Fujimuro M, Yokosawa H (2005) Production of antipolyubiquitin monoclonal antibodies and their use for characterization and isolation of polyubiquitinated proteins. Methods Enzymol 399 75-86.