OTUD1 CD(270-481) [6His-tagged]


Catalogue Number
64-0054-050
Product Size
50 µg
Price £
£325
Accession Number
NP_001138845
Residues Expressed
270-481
Certificate of Analysis Size
50 µg
Species
human
Source
E.coli
Quantity
50 µg
Storage
-70°C
Concentration
0.5 mg/ml
Formulation
50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Molecular Weight
~30kDa
Stability
12 months at -70°C; aliquot as required
Protein Sequence
Accession number: NP_001138845. For full protein sequence information download the Certificate of Analysis pdf.
QA; Protein Identification
Confirmed by mass spectrometry.
QA Activity

Deubiquitylase Enzyme Assay: The activity of His-OTUD1 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of His-OTUD1 was compared confirming the deubiquitylating activity of His-OTUD1.