The anti-polyubiquitylated conjugates mAb (FK1) demonstrates specific recognition for polyubiquitin protein conjugates but shows no reactivity with monoubiquitylated proteins or free ubiquitin (Fujimoro et al. 1994). The antipolyubiquitylated conjugates mAb (FK1) has been extensively characterised and used not only to investigate polyubiquitin chain formation on proteins by Western blotting but also in the detection of intracellular polyubiquitin chains in immunoassays (Takada et al. 1995; Fujimoro et al. 2005).
Fujimuro M, Sawada H, Yokosawa H (1994) Production and characterization of monoclonal antibodies specific to multiubiquitin chains of polyubiquitinated proteins. FEBS Lett 349 173-180.
Takada K, Nasu H, Hibi N, Tsukada Y, Ohkawa K, Fujimuro M,Sawada H, Yokosawa H (1995) Immunoassay for the quantification of intracellular multi-ubiquitin chains. Eur J Biochem 233 42-47.
Fujimuro M, Yokosawa H (2005) Production of antipolyubiquitin monoclonal antibodies and their use for characterization and isolation of polyubiquitinated proteins. Methods Enzymol 399 75-86.