SMURF2 [GST-tagged]

Catalogue Number
Product Size
25 µg
Price £
Accession Number
Residues Expressed
Certificate of Analysis Size
25 µg
25 µg
50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Molecular Weight
12 months at -70°C; aliquot as required
Protein Sequence
Accession number: AAG45422.1. For full protein sequence information download the Certificate of Analysis pdf.
QA; Protein Identification
Confirmed by mass spectrometry.
QA Activity

E3 Ligase Assay: The ubiquitin conjugating activity of GST-SMURF2 was validated through its ability to catalyse the generation of polyubiquitin chains in the presence of the E1 activating enzyme His-UBE1, the E2 conjugating enzyme His-UBE2D3 (UbcH5c) (several E2s were tested, data generated with this E2 is provided by way of example) and ubiquitin. Incubation of GST-SMURF2 for 60 minutes at 30oC in the presence of ubiquitin, His-UBE1, His-UBE2D3 and ATP (Lane 1) was compared alongside two control reactions with either ATP (Lane 2) or GST-SMURF2 (Lane 3) excluded from the reaction. Ubiquitin conjugates were identified by Western blotting using an anti-ubiquitin conjugate antibody and these were observed only in the presence of both ATP and GST-SMURF2.


The enzymes of the ubiquitylation pathway play a pivotal role in a number of cellular processes including the regulated and targeted proteasome-dependent degradation of substrate proteins. Three classes of enzymes are involved in the process of ubiquitylation; activating enzymes (E1s), conjugating enzymes (E2s) and protein ligases (E3s). Smad-Specific E3 Ubiquitin Protein Ligase 1 (SMURF2) is a member of the E3 protein ligase family and cloning of the human gene was first described by Kavsak et al. (2000). SMURF2 is a HECT domain ubiquitin E3 ligase that has been shown to regulate cell polarity, senescence and tumor suppression (Blank et al., 2012). Immunoprecipitation studies have demonstrated that SMURF2 interacts with RNF11 through the binding of the WW domain 2 and 3 of SMURF2 to the PY motif of RNF11. RNF11 was also found to interact with Ube2D1 in this complex and ubiquitiylation of both SMURF2 and RNF11 was detected. (Subramaniam et al., 2003). Knock down of SMURF2 in human tumour cell lines results in increased levels of RNF20 and ubiquitylation of the RNF20 substrate histone H2B (Blank et al., 2012). SMURF2 knockout mice appear normal until early adulthood, when a large number of them develop tumours of all types (Blank et al., 2012).


Blank M, Tang Y, Yamashita M, Burkett SS, Cheng SY. et al. (2012) A tumor suppressor function of Smurf2 associated with controlling chromatin landscape and genome stability through RNF20. Nature Med. 18, 227-234.

Kavsak P, Rasmussen RK, Causing CG, Bonni S. et al. (2000) Smad7 binds to Smurf2 to form an E3 ubiquitin ligase that targets the TGF-beta receptor for degradation. Molec Cell 6, 1365-1375.

Subramaniam V, Li H, Wong M, Kitching R, Attisano L, Wrana J, Zubovits J, Burger AM, Seth A. (2003) The RING-H2 protein RNF11 is overexpressed in breast cancer and is a target of Smurf2 E3 ligase. Brit J Cancer 89, 1538-1544.