UBE2J2 (NCUBE2) residues (1-226) [untagged]


Catalogue Number
62-0067-100
Product Size
100 µg
Price £
£325
Accession Number
NP_477515.2
Residues Expressed
1-226
Alternate Product Size
20 µg
Certificate of Analysis Size
100 µg
Species
Human
Source
E. coli expression
Quantity
100 μg
Storage
-70°C
Concentration
1 mg/ml
Formulation
50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Molecular Weight
~27 kDa
Stability
12 months at -70°C; aliquot as required
Protein Sequence
Accession number: NP_477515.2. For full protein sequence information download the Certificate of Analysis pdf.
QA; Protein Identification
Confirmed by mass spectrometry.
QA Activity

E2-Ubiquitin Thioester Loading Assay: The activity of UBE2J2 was validated by loading E1 UBE1 activated ubiquitin onto the active cysteine of the UBE2J2 E2 enzyme via a transthiolation reaction. Incubation of the UBE1 and UBE2J2 enzymes in the presence of ubiquitin and ATP at 30°C was compared at two time points, T0 and T10   minutes. Sensitivity of the ubiquitin/UBE2J2 thioester bond to the reducing agent DTT was confirmed.

 


Background

The enzymes of the ubiquitylation pathway play a pivotal role in a number of cellular processes including regulated and targeted proteasomal degradation of substrate proteins. Three classes of enzymes are involved in the process of ubiquitylation; activating enzymes (E1s), conjugating enzymes (E2s) and protein ligases (E3s). UBE2J2 is a member of the E2 conjugating enzyme family and the human gene was first described by Lenk et al. (2002). UBE2J2 is a 318 amino acid protein that shares 26% identity to its yeast homologue (Lenk et al., 2002). UBE2J2 is localised to the cytoplasmic surface of the Endoplasmic Reticulum (ER) and participates in Endoplasmic Reticulum Associated Degradation (ERAD). It has been demonstrated that expression of a mutant form of UBE2J2 affects ERAD of the T cell receptor and a mutant form of the CFTR protein (Lenk et al., 2002). UBE2J2 has also been identified as the primary cellular E2 recruited by the E3 ligase murine K3 (mK3), and this E2-E3 pair is capable of conjugating ubiquitin on lysine or serine residues of substrates. Interestingly, UBE2J2-mK3 preferentially promotes ubiquitylation of hydroxylated amino acids via ester bonds even when lysine residues are present on wild-type substrates (Wang et al., 2009). Treatment of Sertoli and germ cells of the testies with a proteasome inhibitor results in the colocalisation of the retinoic acid receptor alpha (RARA) to the ER where it interacts with UBE2J2 resulting in its ubiquitylation and degradation via the ERAD pathway (Zhu et al., 2010).


References

Lenk U, Yu H, Walter J, Gelman MS, Hartmann E, Kopito RR, Sommer T (2002) A role for mammalian Ubc6 homologues in ER-associated protein degradation. J Cell Sci 115, 3007-14.

Wang X, Herr RA, Rabelink M, Hoeben RC, Wiertz EJ, Hansen TH (2009) Ube2j2 ubiquitinates hydroxylated amino acids on ER-associated degradation substrates. J Cell Biol 187, 655-68.

Zhu L, Santos NC, Kim KH (2010) Disulfide isomerase glucose-regulated protein 58 is required for the nuclear localization and degradation of retinoic acid receptor alpha. Reproduction 139(4), 717-31.