*Purity of all products >95% unless otherwise stated
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NameCatalogue NumberSizePriceAdd to Basket
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Catalogue Number:60-0122-500Size:500 µlPrice:£200Add To Basket
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Specieshuman
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Sourcesynthetic
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Quantity500 µl
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Storage-70°C
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Concentration0.4µM
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FormulationDTT containing buffer
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Molecular Weight~8.93kDa
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Stability12 months at -70°C. Avoid multiple freeze/thaw cycles.
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Protein SequenceAccession number: P62987. C-terminally tagged with Rhodamine 110
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QA; Protein IdentificationConfirmed by mass spectrometry.
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QA; ActivityActivity Assay: The activity of Ubiquitin-Rhodamine 110 was validated by determining the increase in fluorescence at 535nm (Excitation 485nm) measured as a result of the enzyme catalysed cleavage at the amide bond between the C-terminal Glycine and Rhodamine, generating Ubiquitin and de-quenched Rhodamine 110-Glycine. UCHL3 (deubiquitylase) was incubated with Ubiquitin-Rhodamine 110 and the fluorescence was measured at four time points (0min, 30min, 60min and 90min).
see Certificate of Analysis