Enabling Protein Degradation Drug Discovery

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  • Name
    Catalogue Number
    Size
    Price
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  • Name:
    4x Ubiquitin-Rhodamine 110
    Catalogue Number:
    60-0122-500
    Size:
    500 µl
    Price:
    £200
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  • Species
    human
  • Source
    synthetic
  • Quantity
    500 µl
  • Storage
    -70°C
  • Concentration
    0.4µM
  • Formulation
    DTT containing buffer
  • Molecular Weight
    ~8.93kDa
  • Stability
    12 months at -70°C. Avoid multiple freeze/thaw cycles.
  • Protein Sequence
    Accession number: P62987. C-terminally tagged with Rhodamine 110
  • QA; Protein Identification
    Confirmed by mass spectrometry.
  • QA; Activity
    Activity Assay: The activity of Ubiquitin-Rhodamine 110 was validated by determining the increase in fluorescence at 535nm (Excitation 485nm) measured as a result of the enzyme catalysed cleavage at the amide bond between the C-terminal Glycine and Rhodamine, generating Ubiquitin and de-quenched Rhodamine 110-Glycine. UCHL3 (deubiquitylase) was incubated with Ubiquitin-Rhodamine 110 and the fluorescence was measured at four time points (0min, 30min, 60min and 90min).

see Certificate of Analysis